- Identity
- HPTLC fingerprint or microscopy to confirm Tribulus terrestris and the plant part (fruit or aerial parts).
- Saponin or protodioscin assay
- Total saponins by gravimetric or UV method, or protodioscin by HPLC; the COA should name the method, since results differ widely between them.
- Heavy metals
- Lead, arsenic, cadmium and mercury by ICP-MS, checked against the buyer's limits and any Prop 65 exposure targets.
- Microbiology
- Total plate count, yeast and mold, coliforms or E. coli, and Salmonella. Raw powders carry more microbial load than extracts and are often steam treated.
- Residual solvents
- For extracts made with ethanol or other solvents, residual solvents against USP <467> or the buyer's specification.
- Physical tests
- Loss on drying, mesh size, bulk density and the carrier, if any.