- Identity
- Microscopy, HPTLC fingerprint or DNA barcoding to confirm Hericium erinaceus and the material type (fruiting body or mycelium).
- Beta-glucan and alpha-glucan
- Beta-glucans by an enzymatic method (such as the Megazyme mushroom and yeast assay) alongside alpha-glucan or starch. High starch points to grain substrate; total polysaccharide by colorimetric methods counts starch too, so the spec should name the method.
- Marker compounds
- Erinacine A by HPLC for mycelium ingredients sold on that basis; hericenones are sometimes reported for fruiting body extracts.
- Heavy metals
- Lead, arsenic, cadmium and mercury by ICP-MS. Mushrooms take up metals from their substrate, so every lot is checked.
- Microbiology
- Total plate count, yeast and mold, E. coli and Salmonella.
- Pesticides and residual solvents
- Multi-residue pesticide screen (required for organic) and residual ethanol for dual extracts.
- Physical tests
- Loss on drying, mesh size, solubility and the extraction ratio or carrier, if any.