- Identity (HPTLC)
- Fingerprint against Vitex agnus-castus fruit, distinguishing it from other Vitex species.
- Marker assay (HPLC)
- Agnuside and/or casticin by a named HPLC method.
- Residual solvents
- For extracts, results against USP <467> or the buyer's spec.
- Heavy metals
- Lead, arsenic, cadmium and mercury by ICP-MS, checked against the buyer's limits and any Prop 65 exposure targets.
- Microbiology
- Total plate count, yeast and mold, E. coli and Salmonella (often Staphylococcus aureus too).
- Pesticide residues
- Multi-residue screen by GC-MS/MS and LC-MS/MS, required for organic claims and commonly requested for conventional material.