- Identity (macroscopy, microscopy, HPTLC)
- Checks to confirm Cnicus benedictus and flag milk thistle (Silybum marianum) or other thistles sold under similar names.
- Marker or bitterness test
- Cnicin by HPLC or a bitterness value, where the specification sets one.
- Heavy metals
- Lead, arsenic, cadmium and mercury by ICP-MS, checked against the buyer's limits and any Prop 65 exposure targets.
- Microbiology
- Total plate count, yeast and mold, E. coli or coliforms, and Salmonella.
- Pesticide residues
- Multi-residue pesticide screen, required for organic claims and commonly requested for conventional material.
- Physical tests
- Loss on drying, mesh or particle size, bulk density and, for extracts, the extract ratio, carrier and residual solvent.